蚯蚓溶解酶凍干機：蚯(qiu)蚓(yin)纖溶(rong)(rong)(rong)酶(mei)(mei)具(ju)有直(zhi)接水解纖維蛋(dan)白和(he)激活血纖維蛋(dan)白溶(rong)(rong)(rong)酶(mei)(mei)原成血纖維蛋(dan)白溶(rong)(rong)(rong)酶(mei)(mei)，有抗栓和(he)溶(rong)(rong)(rong)栓作用(yong)(yong)。生物(wu)凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)機(ji)設備是(shi)利(li)用(yong)(yong)生物(wu)真空冷凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)燥技術，進(jin)行各種生物(wu)制品凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)、人參提取(qu)凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)、蛋(dan)白酶(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)、益生菌凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)、龜膏凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)、多(duo)肽凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)、花青素凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)、蚯(qiu)蚓(yin)纖溶(rong)(rong)(rong)酶(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)等加工(gong)(gong)生產。其(qi)中蚯(qiu)蚓(yin)纖溶(rong)(rong)(rong)酶(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)粉制作工(gong)(gong)藝(yi)(yi)是(shi)經過一系列(lie)的前處理后采用(yong)(yong)真空冷凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)燥工(gong)(gong)藝(yi)(yi)，得到原有營(ying)養成分(fen)和(he)活性物(wu)質的蚯(qiu)蚓(yin)纖溶(rong)(rong)(rong)酶(mei)(mei)凍(dong)(dong)(dong)(dong)(dong)(dong)干(gan)(gan)(gan)粉。

蚯蚓纖溶酶凍干(gan)粉制造工藝具體(ti)如下：

1）制(zhi)作(zuo)蚯(qiu)蚓(yin)粗粉：將活(huo)蚯(qiu)蚓(yin)饑餓(e)自然吐凈泥沙，清洗，風(feng)干后低溫(wen)粉碎，再進行(xing)過(guo)篩制(zhi)成蚯(qiu)蚓(yin)粗粉；

  2）制作粗(cu)酶溶(rong)液(ye)(ye)：將蚯蚓粗(cu)粉用磷(lin)酸(suan)緩沖(chong)液(ye)(ye)PBS和氯化(hua)鈉溶(rong)液(ye)(ye)均勻后(hou)置(zhi)于2~8℃冷柜(ju)靜(jing)(jing)置(zhi)12小(xiao)時，在經過離心，得到上(shang)(shang)清(qing)液(ye)(ye)。再(zai)將上(shang)(shang)清(qing)液(ye)(ye)進(jin)行(xing)水浴(yu)處理后(hou)進(jin)行(xing)第(di)二次離心取上(shang)(shang)清(qing)液(ye)(ye)，在上(shang)(shang)清(qing)液(ye)(ye)中加硫酸(suan)銨(an)達靜(jing)(jing)置(zhi)后(hou)進(jin)行(xing)第(di)三次離心，得到蚯蚓纖溶(rong)酶粗(cu)酶溶(rong)液(ye)(ye)；

3）粗(cu)酶(mei)(mei)純化和(he)(he)修(xiu)(xiu)飾(shi)：將蚯蚓纖溶(rong)(rong)酶(mei)(mei)粗(cu)酶(mei)(mei)溶(rong)(rong)液過Sephadex G75凝(ning)膠層析，經(jing)過沖(chong)(chong)液洗脫得到蚯蚓纖溶(rong)(rong)酶(mei)(mei)純酶(mei)(mei)。再(zai)加入活化的PEG修(xiu)(xiu)飾(shi)劑(ji)和(he)(he)硼酸緩(huan)沖(chong)(chong)液，進行保溫反應，在經(jing)過磷酸緩(huan)沖(chong)(chong)液透析和(he)(he)離心進行上清(qing)液純化，收集活性成分得到蚯蚓纖溶(rong)(rong)酶(mei)(mei)修(xiu)(xiu)飾(shi)酶(mei)(mei)；

4）蚯(qiu)蚓(yin)纖(xian)溶酶冷凍(dong)干(gan)(gan)(gan)燥(zao)：將(jiang)蚯(qiu)蚓(yin)纖(xian)溶酶修飾酶裝盤，料厚8mm左(zuo)右，進行-45℃低溫(wen)冷凍(dong)300min，而后在35pa真空下(xia)，進行一次干(gan)(gan)(gan)燥(zao)控(kong)(kong)溫(wen)范圍-30~10℃，升華干(gan)(gan)(gan)燥(zao)時間(jian)720min,除去大部分流離(li)水，然后進行解析干(gan)(gan)(gan)燥(zao)控(kong)(kong)溫(wen)范圍10~40℃，干(gan)(gan)(gan)燥(zao)運行時間(jian)860min。經中(zhong)生物凍(dong)干(gan)(gan)(gan)機的終點判斷確定，凍(dong)干(gan)(gan)(gan)結束得到含水1%以內的蚯(qiu)蚓(yin)纖(xian)溶酶凍(dong)干(gan)(gan)(gan)粉。